Ischemia-reperfusion injury of the mouse kidney.

Document Type

Article

Publication Date

1-1-2012

Institution/Department

Maine Medical Center Research Institute

Journal Title

Methods in molecular biology (Clifton, N.J.)

MeSH Headings

Anesthetics, General, Animals, Disease Models, Animal, Female, Hypnotics and Sedatives, Isoflurane, Ketamine, Kidney, Male, Mice, Nephrons, Reperfusion Injury, Xylazine

Abstract

Studies of the complex responses of the kidney to acute injury have yielded important insights into mechanisms of tissue injury and repair. A variety of injury models have contributed to this impressive body of knowledge, but the ischemia-reperfusion (IR) model has perhaps been the most widely used. This chapter contains a detailed method description for IR injury in the mouse together with notes on blood sampling and tissue harvesting. The aim of the chapter is to provide the novice with a step-by-step guide to establishing this procedure in their research program.

ISSN

1940-6029

First Page

363

Last Page

379

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