Title

Overview of genetic tools and techniques to study Notch signaling in mice.

Document Type

Article

Publication Date

1-1-2014

Institution/Department

Molecular Medicine, MMCRI

Journal Title

Methods in molecular biology (Clifton, N.J.)

MeSH Headings

Animals, Breeding, Genetic Techniques, Integrases, Mice, Mutation, Receptors, Notch, Recombinant Fusion Proteins, Signal Transduction, Tamoxifen

ISSN

1940-6029

Abstract

Aberrations of Notch signaling in humans cause both congenital and acquired defects and cancers. Genetically engineered mice provide the most efficient and cost-effective models to study Notch signaling in a mammalian system. Here, we review the various types of genetic models, tools, and strategies to study Notch signaling in mice, and provide examples of their use. We also provide advice on breeding strategies for conditional mutant mice, and a protocol for tamoxifen administration to mouse strains expressing inducible Cre recombinase-estrogen receptor fusion proteins.

First Page

47

Last Page

61

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