Maine Medical Center, Medical Education, Maine Medical Center Research Institute
Multiple Myeloma, FABP4 protein, human, Fatty Acid-Binding Proteins, Cell Proliferation
In the bone marrow microenvironment, adipocytes and multiple myeloma cells have an intricate bidirectional relationship. Adipokines have been linked to shifting typical behavior in many cancers such as proliferation. Our previous research found that Fatty Acid Binding Protein (FABP) family expression changes in the presence of bone marrow adipocytes. To test the effect that FABPs may have on myeloma cell growth, a cell line was treated with inhibitors of FABP4, FABP5, and a co-treatment in cell culture. Bioluminescence imaging (BLI) was used to cell count the myeloma cells at time 0, 24, 48, and 72 hours. Analysis of the inhibitor spike in showed that myeloma cell growth was inhibited over time with the individual inhibitors and the co-inhibitor treatment. To test if external FABPs are important to myeloma cells, recombinant FABP4 or FABP5 was spiked into myeloma cell lines (MM1.S and OPM2) in serum and serum-free conditions. This data was cell counted using BLI. Analysis showed that there was no impact on cell growth with the spike in. Thus, external FABPs are not important to myeloma cells. A future direction is to test the effect that inhibiting internal FABPs may have on myeloma cells by knocking down FABP5 by using methods of lipofectamine transfection and nucleofection to transfect siRNA. The successful inhibition of FABP4, FABP5, or both may prove useful as a potential cancer therapeutic to stop multiple myeloma cell proliferation in the human body.
D’Amico, Anastasia; Farrell, Mariah; and Reagan, Michaela, "Investigating the Effect that Inhibiting Fatty Acid Binding Proteins 4 or 5 Have on Myeloma Cell Proliferation" (2020). Costas T. Lambrew Research Retreat 2020. 44.