Long-Term Culture of Nephron Progenitor Cells Ex Vivo.

Document Type

Article

Publication Date

1-1-2019

Institution/Department

Maine Medical Center Research Institute, Center for Molecular Medicine

Journal Title

Methods in molecular biology (Clifton, N.J.)

MeSH Headings

Animals, Cell Differentiation, Cells, Cultured, Humans, Kidney, Mice, Organogenesis, Stem Cells

Abstract

Nephrons differentiate from the cap mesenchyme of the fetal kidney. Nephron progenitor cells that populate the cap mesenchyme efficiently balance self-renewal and epithelial differentiation to enable repeated rounds of nephron formation during development. Here we describe a method to isolate and propagate these cells from the embryonic mouse kidney. Using this method, nephron progenitor cells from a single litter of mice can be propagated to hundreds of millions of cells that express appropriate markers of the undifferentiated state and retain epithelial differentiation capacity in vitro.

ISSN

1940-6029

First Page

63

Last Page

75

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