Long-Term Culture of Nephron Progenitor Cells Ex Vivo.
Maine Medical Center Research Institute, Center for Molecular Medicine
Methods in molecular biology (Clifton, N.J.)
Animals, Cell Differentiation, Cells, Cultured, Humans, Kidney, Mice, Organogenesis, Stem Cells
Nephrons differentiate from the cap mesenchyme of the fetal kidney. Nephron progenitor cells that populate the cap mesenchyme efficiently balance self-renewal and epithelial differentiation to enable repeated rounds of nephron formation during development. Here we describe a method to isolate and propagate these cells from the embryonic mouse kidney. Using this method, nephron progenitor cells from a single litter of mice can be propagated to hundreds of millions of cells that express appropriate markers of the undifferentiated state and retain epithelial differentiation capacity in vitro.
Brown, Aaron C; Gupta, Ashwani K; and Oxburgh, Leif, "Long-Term Culture of Nephron Progenitor Cells Ex Vivo." (2019). Maine Medical Center. 1453.