Title

FOXD1 promotes nephron progenitor differentiation by repressing decorin in the embryonic kidney.

Document Type

Article

Publication Date

1-1-2014

Institution/Department

Molecular Medicine

Journal Title

Development (Cambridge, England)

MeSH Headings

Animals, Bone Morphogenetic Protein 7, Cell Differentiation, Cell Line, Decorin, Forkhead Transcription Factors, Gene Expression Profiling, Lymphoid Enhancer-Binding Factor 1, Mice, Mice, Inbred ICR, Mice, Knockout, NIH 3T3 Cells, Nephrons, Nuclear Proteins, Organogenesis, Signal Transduction, Smad Proteins, Stem Cells, Trans-Activators, Transcriptome

ISSN

1477-9129

Abstract

Forkhead transcription factors are essential for diverse processes in early embryonic development and organogenesis. Foxd1 is required during kidney development and its inactivation results in failure of nephron progenitor cell differentiation. Foxd1 is expressed in interstitial cells adjacent to nephron progenitor cells, suggesting an essential role for the progenitor cell niche in nephrogenesis. To better understand how cortical interstitial cells in general, and FOXD1 in particular, influence the progenitor cell niche, we examined the differentiation states of two progenitor cell subtypes in Foxd1(-/-) tissue. We found that although nephron progenitor cells are retained in a primitive CITED1-expressing compartment, cortical interstitial cells prematurely differentiate. To identify pathways regulated by FOXD1, we screened for target genes by comparison of Foxd1 null and wild-type tissues. We found that the gene encoding the small leucine-rich proteoglycan decorin (DCN) is repressed by FOXD1 in cortical interstitial cells, and we show that compound genetic inactivation of Dcn partially rescues the failure of progenitor cell differentiation in the Foxd1 null. We demonstrate that DCN antagonizes BMP/SMAD signaling, which is required for the transition of CITED1-expressing nephron progenitor cells to a state that is primed for WNT-induced epithelial differentiation. On the basis of these studies, we propose a mechanism for progenitor cell retention in the Foxd1 null in which misexpressed DCN produced by prematurely differentiated interstitial cells accumulates in the extracellular matrix, inhibiting BMP7-mediated transition of nephron progenitor cells to a compartment in which they can respond to epithelial induction signals.

First Page

17

Last Page

27

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