Title

MCT8 deficiency in male mice mitigates the phenotypic abnormalities associated with the absence of a functional type 3 deiodinase.

Document Type

Article

Publication Date

8-2016

Institution/Department

Molecular Medicine, MMCRI

Journal Title

Endocrinology

MeSH Headings

Animals, Animals, Newborn, Fetal Growth Retardation, Fetal Viability, Growth and Development, Hypothalamus, Hypothyroidism, Iodide Peroxidase, Male, Membrane Transport Proteins, Mice, Mice, Knockout, Phenotype, Thyroid Gland

ISSN

1945-7170

Abstract

Mice deficient in the type 3 deiodinase (D3KO mice) manifest impaired clearance of thyroid hormone (TH), leading to elevated levels of TH action during development. This alteration causes reduced neonatal viability, growth retardation, and central hypothyroidism. Here we examined how these phenotypes are affected by a deficiency in the monocarboxylate transporter 8 (MCT8), which is a major contributor to the transport of the active thyroid hormone, T3, into the cell. MCT8 deficiency eliminated the neonatal lethality of type 3 deiodinase (D3)-deficient mice and significantly ameliorated their growth retardation. Double-mutant newborn mice exhibited similar peripheral thyrotoxicosis and increased brain expression of T3-dependent genes as mice with D3 deficiency only. Later in neonatal life and adulthood, double-mutant mice manifested central and peripheral TH status similar to mice with single MCT8 deficiency, with low serum T4, elevated serum TSH and T3, and decreased T3-dependent gene expression in the hypothalamus. In double-mutant adult mice, both thyroid gland size and the hypothyroidism-induced rise in TSH were greater than those in mice with single D3 deficiency but less than those in mice with MCT8 deficiency alone. Our results demonstrate that the marked phenotypic abnormalities observed in the D3-deficient mouse, including perinatal mortality, growth retardation, and central hypothyroidism in adult animals, require expression of MCT8, confirming the interdependent relationship between the TH transport into cells and the deiodination processes.

First Page

3266

Last Page

3277

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