Parathyroid hormone directs bone marrow mesenchymal cell fate.
Adipocytes, Adipogenesis, Adipose Tissue, Animals, Biomarkers, Bone Marrow Cells, Bone and Bones, Cell Count, Cell Lineage, Humans, Integrases, Male, Mesenchymal Stem Cells, Mice, Osteoblasts, Osteoporosis, Parathyroid Hormone, Phenotype, RANK Ligand, Receptor, Parathyroid Hormone, Type 1, Signal Transduction, Skull
Intermittent PTH administration builds bone mass and prevents fractures, but its mechanism of action is unclear. We genetically deleted the PTH/PTHrP receptor (PTH1R) in mesenchymal stem cells using Prx1Cre and found low bone formation, increased bone resorption, and high bone marrow adipose tissue (BMAT). Bone marrow adipocytes traced to Prx1 and expressed classic adipogenic markers and high receptor activator of nuclear factor kappa B ligand (Rankl) expression. RANKL levels were also elevated in bone marrow supernatant and serum, but undetectable in other adipose depots. By cell sorting, Pref1
Fan, Yi; Hanai, Jun-Ichi; Le, Phuong T; Bi, Ruiye; Maridas, David; DeMambro, Victoria; Figueroa, Carolina A; Kir, Serkan; Zhou, Xuedong; Mannstadt, Michael; Baron, Roland; Bronson, Roderick T; Horowitz, Mark C; Wu, Joy Y; Bilezikian, John P; Dempster, David W; Rosen, Clifford J; and Lanske, Beate, "Parathyroid hormone directs bone marrow mesenchymal cell fate." (2017). Maine Medical Center. 634.