Protein-phospholipid interactions in nonclassical protein secretion: problem and methods of study.

Document Type

Article

Publication Date

2-8-2013

Institution/Department

Maine Medical Center Research Institute

Journal Title

Int J Mol Sci

MeSH Headings

Phospholipids, Protein Transport, Biophysical Phenomena

Abstract

Extracellular proteins devoid of signal peptides use nonclassical secretion mechanisms for their export. These mechanisms are independent of the endoplasmic reticulum and Golgi. Some nonclassically released proteins, particularly fibroblast growth factors (FGF) 1 and 2, are exported as a result of their direct translocation through the cell membrane. This process requires specific interactions of released proteins with membrane phospholipids. In this review written by a cell biologist, a structural biologist and two membrane engineers, we discuss the following subjects: (i) Phenomenon of nonclassical protein release and its biological significance; (ii) Composition of the FGF1 multiprotein release complex (MRC); (iii) The relationship between FGF1 export and acidic phospholipid externalization; (iv) Interactions of FGF1 MRC components with acidic phospholipids; (v) Methods to study the transmembrane translocation of proteins; (vi) Membrane models to study nonclassical protein release.

ISSN

1422-0067

First Page

3734

Last Page

3772

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