"Isolation and Characterization of Primary Bone Marrow Adipocytes in Ro" by Samantha N. Costa, Carolyn Chlebek et al.
 

Isolation and Characterization of Primary Bone Marrow Adipocytes in Rodent Models

Document Type

Article

Publication Date

1-2025

Institution/Department

Center for Molecular Medicine

Journal Title

Methods in molecular biology (Clifton, N.J.)

MeSH Headings

Animals; Adipocytes (cytology, metabolism); Bone Marrow Cells (cytology); Cell Separation (methods); Mice; Rats; Cells, Cultured; Cell Differentiation

Abstract

This chapter will present innovative methods for the isolation and characterization of mature, primary bone marrow adipocytes from rodents. The key advantage of this protocol is that it allows for ex vivo analyses of mature bone marrow adipocytes, which is a more direct approach than traditional in vitro studies. While the difficulty in isolating mature adipocytes from the heterogenous bone marrow population has precluded their characterization in many preclinical studies, we have optimized our methods to maximize the collection of alive, mature bone marrow adipocytes and minimize contamination of other bone marrow and immune cells.

First Page

87

Last Page

98

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