Title

Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model.

Document Type

Article

Publication Date

1-1-2016

Institution/Department

Center for Molecular Medicine, Maine Medical Center Research Institute

Journal Title

Methods in molecular biology (Clifton, N.J.)

MeSH Headings

Animals, Cell Line, Tumor, Cell Transformation, Neoplastic, Chick Embryo, Chorioallantoic Membrane, Humans, Neovascularization, Pathologic, X-Ray Microtomography

Abstract

Models of tumor angiogenesis have played a critical role in understanding the mechanisms involved in the recruitment of vasculature to the tumor mass, and have also provided a platform for testing antiangiogenic potential of new therapeutics that combat the development of malignant growth. In this regard, the chorioallantoic membrane (CAM) of the developing chick embryo has proven to be an elegant model for investigation of angiogenic processes. Here, we describe methods for effectively utilizing the preestablished vascular network of the chick CAM to investigate and quantify tumor-associated angiogenesis in a breast tumor model.

ISSN

1940-6029

First Page

255

Last Page

269

Share

COinS